Figure 7

Stat6 silencing prevents the induction of differentiation by progesterone. Synchronized T47D cells transfected with control or Stat6 or siRNA (500 ng) and treated with progesterone (30 nM) or vehicle were harvested after 12 h (A) or 4 days (B and C) for quantitative RT-PCR analyses (A and B) and Oil Red O staining for lipid droplets (C). In panels A and B, mRNA levels are expressed relative to vehicle-incubated control siRNA-transfected cells, which were arbitrarily set as 1. NS, not significant; *, P ≤ 0.05; **, P ≤0.01; ***, P ≤ 0.001 versus control. C . Lipid detection. Left panel: representative Oil Red O-stained cellular sections. Right panel: quantification of the lipid staining intensity.