Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis

Figure 1

Process of retrieving target genes of several miRNAs. A) Homology of the sequence between miRNA and TNRC6B. Complementary of the sequence between miR-18b and TNRC6B gene by miRanda algorithm (upper side) and Targetscan algorithm (lower side). B) Changes in TNRC6B expression when miRNA is over-expressed or suppressed are shown means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05). C) Transfection of reporter vectors with either the wild (left part) or mutated (right part) TNRC6B 3UTR and miR-18b. The data shown are means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05). D) Target confirmation by argonaute 2 (Ago2) immunoprecipitation (IP). When miR-18b exists in RISC, compared with existence of control RNA, TNRC6B is abundantly contained in RISC. TNRC6B RNA was measured by real-time qPCR in 10 ng sample of total RNA from the Ago2-IP fraction. The data shown are means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05).

Back to article page