Figure 1From: The expression level of miR-18b in hepatocellular carcinoma is associated with the grade of malignancy and prognosis Process of retrieving target genes of several miRNAs. A) Homology of the sequence between miRNA and TNRC6B. Complementary of the sequence between miR-18b and TNRC6B gene by miRanda algorithm (upper side) and Targetscan algorithm (lower side). B) Changes in TNRC6B expression when miRNA is over-expressed or suppressed are shown means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05). C) Transfection of reporter vectors with either the wild (left part) or mutated (right part) TNRC6B 3′UTR and miR-18b. The data shown are means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05). D) Target confirmation by argonaute 2 (Ago2) immunoprecipitation (IP). When miR-18b exists in RISC, compared with existence of control RNA, TNRC6B is abundantly contained in RISC. TNRC6B RNA was measured by real-time qPCR in 10 ng sample of total RNA from the Ago2-IP fraction. The data shown are means ± SD of three independent experiments. Asterisk indicates a significant difference (p < 0.05).Back to article page