Bag5 increases prostate cancer cell chemoresistance. Modulation of Bag5 levels influences prostate cancer cell response to chemotherapy. A and B. Colony forming assay (A) and caspase-3 cleavage analysis (B) of 22Rv.1 cells stably expressing the empty vector control (open bars) or HA-Bag5 (filled bars). Bar charts represent the average of three independent experiments ± SD. * p < 0.05. (TG: thapsigargin, 75 nM; TN: tunicamycin, 10 μg/ml; EGCG: (-)-epigallocatechingallate, 10 μM; Fen: fenretinide, 20 μM; GS: glucose starvation; SS: serum starvation; Tax: taxol, 10 nM). * p < 0.05; ** p < 0.01. C. Western blot analysis of 22Rv.1 and PC3 cells transfected with siRNA targeting Bag5 or GFP as control and treated with 300 nM TG for 24 hours. Anti-β-actin antibody was used as equal loading control.