Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: Bcl-2 associated athanogene 5 (Bag5) is overexpressed in prostate cancer and inhibits ER-stress induced apoptosis

Figure 1

Bag5 is overexpressed in prostate cancer. Bag5 expression analysis in prostate cancer tissue and cell lines. A. Quantitative RT-PCR studies showing the relative expression of Bag5 in benign prostate hyperplasia (BPH, n = 15) and prostate cancer (PCa, n = 27). The expression values were normalized against the expression of β-actin. The horizontal line indicates arithmetic mean value. Non-parametric Kruskal-Wallis with Dunn’ post-test was used to analyze the statistical significance of the differences between the groups (* = p <0.05). B. Representative immunohistochemical staining used in the tissue array analysis. P63 (blue) and Bag5 (brown) staining are shown. Bening (Be) and cancer (Ca) tissue is indicated. C. Tissue microarray containing tissue cores of 91 cancer cases. The horizontal line indicates arithmetic mean value. (**p < 0.001). D. Western blot analysis of Bag5 protein expression in prostate cancer patients comparing cancer (C) and surrounding benign tissue (B). β-actin was used as equal loading control. E. Western blot analysis of Bag5 protein expression in an in vitro prostate tumor progression model using RWPE-1 (benign), WPE-NB14 (primary tumor) and WPE-NB26 (metastatic tumor) cells. β-actin was used as equal loading control. F. Western blot analysis of Bag5 protein expression in prostate cell lines. Equal loading control was checked with β-actin.

Back to article page