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Figure 4 | BMC Cancer

Figure 4

From: Regional differences in prostaglandin E2 metabolism in human colorectal cancer liver metastases

Figure 4

The effect of hypoxia on NAD+/NADH levels and endogenous 15-PGDH activity in human cancer cells. A) NAD+ and NADH levels in MCF-7 cells cultured in normoxia (20% O2; N) or hypoxia (1% O2; H) for 72 hours. Columns and bars represent the mean and the standard error of the mean (n = 5). B) NAD+ and NADH levels in LIM1863 cells cultured in normoxia (N) or hypoxia (H) for 72 hours. Columns and bars represent the mean and the standard error of the mean (n = 5). C) Ex vivo 15-PGDH activity assay of MCF-7 cell lysates in the presence or absence of exogenous NAD+ in the reaction mix. Columns and bars represent the mean and the standard error of the mean (n = 6).*p < 0.01, **p < 0.01.

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