Effect of HDACi on E-cadherin exon 11 splicing. A. Schematic showing the wild type and exon 11 skipped aberrant transcript. Transcript specific PCR strategy is described in the Material and Methods section. B. Table showing the fold change in E-cadherin wild type RNA expression and exon 11 skipped RNA expression with HDACi treatment. (Data from at least two experiments for ten primary CLL specimens) C. D. Effect of NMD inhibition on HDACi mediated induction of transcripts. Two CLL specimens (CLL#1 and #3) were treated with either emetine alone for 8 hours or with MS-275 (1μM for 48 hours) and in the last 8 hours of MS-275 treatment emetine was added for 8 hours. Bar diagram shows the change in total transcript levels with MS-275 + emetine treatment as compared to the transcript levels in CLL specimens that were treated with emetine only.