Effect of HDAC inhibitors on the E-cadherin transcripts. A. RT-PCR analysis to determine the change in expression of wild type E-cadherin transcripts with HDACi MS-275 treatment. Two primary CLL specimens were treated with two different concentrations of HDACi MS-275 (0.1μM and 1.0μM for 48 hours). (Data from two experiments, mean and SD). B. Western blot analysis of two CLL specimens that were treated with HDACi MS-275 (1μM for 48 hours). Lysates analyzed for acetylated histones H3 (Ac-H3), acetylated histone H4 antibodies (Ac-H4) with antibodies that recognize the acetylated histones and actin control.