Effect of ABZ, 2ME and combination on VEGF levels in tumor and plasma sample. A, Four tumors from each treatment group were analyzed for VEGF levels and the blot is representative of four samples. β-actin was used as a control for equal protein loading. Graph shows densitometric analysis of VEGF immunoblot and each column represents the mean ± SEM. B, Following euthanasia, blood was collected by cardiac puncture and plasma samples were subjected to ELISA assay for VEGF levels. Each column represents mean VEGF levels ± SEM (n = 8–9). (**P < 0.001 compared with single agents).