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Figure 4 | BMC Cancer

Figure 4

From: Negative transcriptional control of ERBB2 gene by MBP-1 and HDAC1: diagnostic implications in breast cancer

Figure 4

In vivo recruitment of MBP-1 and HDAC1 proteins to ERBB2 and c-MYC promoters . (A) Immunoblot analysis of SKBr3 cells transfected with pFlag-MBP-1 or mock-transfected using anti-Flag, anti-HDAC1 and anti-beta-actin antibodies. (B) MBP-1 and HDAC1 occupancy at ERBB2 and c-MYC promoter DNA of input and immunoprecipitated chromatin samples was amplified using primers directed to ERBB2 promoter (ERP2/3), to c-MYC. P2 promoter (MP3/4) and primers directed to an unrelated region of the c-MYC gene (MD). (C, D) Quantification by real-time PCR of chromatin immunoprecipitated with anti-HDAC1 and anti-AcH4 antibodies. The amount of immunoprecipitated DNA was calculated relative to that present in total input chromatin (% input). Each data point is the average of triplicates from three independent ChIP experiments ± SD, p value (* P<0.01) indicate statistical significance.

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