Figure 3

MBP-1 interacts in vivo with ERBB2 and c-MYC promoters . (A) Identification of in vivo binding regions for MBP-1. DNA of input and immunoprecipitated chromatin samples was amplified using primers directed to the ERBB2 promoter region (ERP1/2, ERP2/3 and ERP5/7); primers targeted to the c-MYC P2 promoter (MP3/4) as a positive control; and primers directed to an unrelated region of the c-MYC gene (MD). Numbers indicate the length of the amplified DNA fragments. Reactions in absence of input DNA were included as negative controls (n.c.). (B) Quantification of immunoprecipitated chromatin by real-time PCR. The amount of immunoprecipitated DNA was calculated relative to that present in total input chromatin (% input). Gene-specific PCR detected in vivo binding of MBP-1 to both ERBB2 and c-MYC promoters. Each data point is the average of triplicates from three independent ChIP experiments ± SD and p values (* P< 0.05, § P<0.01) indicate statistical significance.