Figure 4

Knock-down of endogenous PRL-3 expression in A2780 cells upregulates expression of integrin α2 and paxillin. (A) Lysates prepared from the indicated cell lines were examined for various proteins and their phospho-isoforms by immunoblot. GAPDH was used as a loading control. (B) Total RNA was extracted from the indicated cell lines and used for RT-PCR assay with the integrin α2-, integrin β1-, or paxilin-specific primer pairs. GAPDH mRNA was used as a loading control. (C) Profuse and enhanced expression of integrin α2 (green) and paxillin (red) were detected in A2780 PRL-3 KD cells by indirect immunofluorescence staining. To-pro-3 iodide was used to stain DNA (blue). (D) A significant negative correlation between PRL-3 and integrin α2 (ITGA2) mRNA expression levels was observed in primary ovarian cancer specimens from the GSE9891 patient cohort (n = 285, Spearman’s rank test, r = −0.193, p = 0.001).