Sub-cytotoxic MJ down-regulated the expression of MMP-14 in gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were incubated with sub-cytotoxic concentrations of MJ as indicated. A and B, western blot and real-time quantitative RT-PCR indicated that administration of sub-cytotoxic (0.1 and 0.2 mM) MJ to SGC-7901 and MKN-45 cells for 24 hrs resulted in a decrease in the expression of MMP-14, but not of MMP-7 or MMP-9, when compared to that of solvent-treated (mock) cells. C and D, western blot and real-time quantitative RT-PCR indicated that administration of 0.2 mM MJ to SGC-7901 and MKN-45 cells for 6, 12, and 24 hrs, resulted in the decrease of MMP-14 expression in a time-dependent manner, than that of mock cells. The symbol (*) indicates a significant decrease from mock.