Sub-cytotoxic MJ attenuated the migration, invasion and angiogenesis, but not the viabilities or proliferation, of gastric cancer cells. Human gastric cancer cell lines SGC-7901 and MKN-45 were incubated with different concentrations of MJ as indicated. A, MTT colorimetric assay indicated that MJ suppressed the cell viabilities of gastric cancer cells with a range of concentrations (0.5 to 2.0 mM), while lower concentrations of MJ (0.05 to 0.2 mM) exerted no obvious cytotoxicity, when compared to those of solvent-treated (mock) cells. B, EdU incorporation assay revealed that sub-cytotoxic MJ (0.05, 0.1 and 0.2 mM) did not attenuate the proliferation of SGC-7901 and MKN-45 cells, when compared to that of mock cells. C, in scratch migration assay, administration of 0.05, 0.1 and 0.2 mM MJ attenuated the migration capabilities of SGC-7901 and MKN-45 cells in a dose- and time-dependent manner, when compared to those of mock cells. D, transwell analysis indicated that administration of sub-cytotoxic MJ (0.05, 0.1 and 0.2 mM) impaired the invasion capacities of SGC-7901 and MKN-45 cells in a dose- and time-dependent manner, than those of mock cells. E, the tube formation of endothelial cells was dose- and time-dependently suppressed by the medium preconditioned by treatment of gastric cancer cells with sub-cytotoxic MJ (0.05, 0.1 and 0.2 mM), than that of mock cells. The symbol (*) indicates a significant decrease from mock.