Figure 4
From: Multicellular tumor spheroid models to explore cell cycle checkpoints in 3D
Lovastatin-induced cell cycle arrest in G1. (A): Capan-2 spheroids treated or not with 10 μM lovastatin for 48 h. Visualization of Fucci-red and Fucci-green expressing cells. Nuclei are stained with DAPI (blue). Scale bar: 50 μm. (B) Capan-2 spheroids treated or not with 10 μM lovastatin for 24 or 48 h. Quantification of the percentage of Fucci-red and Fucci-green expressing cells as a function of the distance to the spheroid surface.