Figure 6

Western blot analysis of TUBB3 and HuR expression on A2780, OVCAR-3 and OV2774 cells untrasfected (“untr” lanes) or transfected with siC and siHuR, as indicated. Cells were cultured in normoglycemia (+) or in hypoglycemia (−) for 48 hours (for methods details, see [16]). The asterisk indicated the mitochondrial form of TUBB3. Actin served as loading control. Densitometric analysis of the TUBB3 bands corresponding to the TUBB3 cytoplasmatic form is shown on the bottom side of the panels and values are expressed as percentage relative to the untreated controls.