A: The scheme above represented the TUBB3 30UTR region, with the miR-200c binding site and the HuR binding region, according to [[12, 27]]. The schematic representations of the S1-tagged constructs were shown below, as the method utilized for the purification of the mRNP complexes. B: qPCR analysis of the luciferase expression in A2780 cells stably transfected with UTR-S1 or UTRm-S1 vectors and cultured in hypoglycemia for 48 hours (− glucose), in comparison with the expression in normoglycemia (+ glucose). C: qPCR analysis of the exogenous mRNAs recovery (LUC), upon mRNA/RBP complexes purification as indicated in panel A. A2780 cells expressing either UTR-S1 or UTRm-S1 vectors were cultured in hypoglycemia (−) or in normoglycemia (+) for 48 hours. The values are expressed as percentage relative to input mRNAs. As control also the recovery of RNA polymerase II mRNA was analyzed (RNApol). D: Representative Western blot analysis of Ago2 and HuR proteins in streptavidin-bound complexes (S1-bound) and in extracts not bound to streptavidin (S1-unbound) purified from cell lines as in panel C.