A: Representative western blot analysis of TUBB3 expression on A2780 miR-200c overexpressing clones (200c-5 and 200c-14) relative to A2780 transfected with the empty vector (A2780-pUSE) upon treatment in hypoglycemia for 72 hours (left panels). The same treatment was also performed on OVCAR-3 cells (right panels). The total protein content was used as loading control. The asterisk indicated the mitochondrial form of TUBB3. B: Densitometric analysis of the bands of panel A. Values are expressed as percentage relative to the untreated controls. Bar and error bars represent the mean and SD of two independent experiments. Only in PUSE there was an increase in hypoglycemia of TUBB3 expression which was significant. C: qPCR analysis of miR-200c expression on A2780 and OVCAR-3 cells as indicated, upon treatment in hypoglycemia, conditions for hours. Values are expressed as percentage relative to the untreated controls. In the two cellular models the opposite significant (asterisk=P-value <0.05) trend for miR-200c expression was noticed.