Figure 5

MYBBP1A induces apoptosis during detached conditions in a p53-dependent manner. (A) MCF-7 cells were transfected with siRNA or a plasmid for p53 or MYBBP1A expression for 48 h, followed by anoikis assay. These cells were cultured under suspension conditions for 24 h, and apoptotic cells were determined by Annexin V–FITC/PI staining followed by flow cytometry analyses. (B) p53 is required for MYBBP1A-induced anoikis. As indicated, MCF-7 cells were transfected with a plasmid for MYBBP1A expression with or without siRNA for p53. They were then cultured under suspension conditions for 24 h, after which apoptotic cells were determined by Annexin V–FITC/PI staining and flow cytometry analyses.