MYBBP1A induces anoikis in a p53-dependent manner. (A) and (B) p53 is required for MYBBP1A-induced anoikis. As indicated, MCF-7 and ZR-75-1 cells were transfected with a plasmid for MYBBP1A expression with or without siRNA for p53. These cells were cultured under suspension conditions for 24 h, and viable cell numbers were counted using trypan blue dye exclusion or assessed by MTT assay. Protein levels of MYBBP1A and p53 in the cells were determined by immunoblotting. Bars = mean + s.d. (n = 3).