Table 2 Primer sequences used for methylation analysis
From: Epigenetic modulation of the drug resistance genes MGMT, ABCB1 and ABCG2 in glioblastoma multiforme
Gene symbol | GenBank accession | Forward primer 5′- > 3′ | Reverse primer 5′- > 3′ | Sequencing primer 5′- > 3′ | Amplicon size (bp) |
|---|---|---|---|---|---|
MGMT | X61657.1 | YGYGTTTYGGATATGTTGGGATAG | Biotin -AACRAAA CRACCCAAACACTCA | GGATAGTTYGYGTTTTTAGA | 115 |
ABCB1 | AH002875.1 | GTGGGTGGGAGGAAGTAT | Biotin -AAATCTC CAACATCTCCAC | GGGTAAAGTTTAGAA | 125 |
ABCG2 | AH011213.2 | TGATTGGGTAATTTGTGTGTTAGTG | Biotin -AAATAAA CCAAAATAATTA ACTAC | TTGTGATTGGGTAATTTGTG | 147 |