Table 2 Immunohistochemistry: Antibodies detecting Gd or GdA were published by Jeschke et al. 2006[26] and Jeschke et al. 2005[32], respectively
From: Immunosuppressive Glycodelin A is an independent marker for poor prognosis in endometrial cancer
Antibody | Host/clonality | Epitope retrieval | Blocking | Dilution/incubation | Negative control | Reaction system |
|---|---|---|---|---|---|---|
Gd [32] | Rabbit/polyclonal | Not performed | Reagent 1 (5 min) | 1: 800 (diluted in Dako antibody diluent)/o.n. (4°C) | Rabbit pre-immune serum (Dako) | Vectastain elite (rabbit IgG) kit (Burlingame, CA) |
GdA [26] | Mouse/monoclonal | Citrate buffer (5 min, pressure cooker) | 1.5% horse serum (20 min) | 1:2000 (diluted in Dako antibody diluent)/o.n. (4°C) | Mouse pre-immune serum (Dako) | Vectastain elite (mouse IgG) kit (Burlingame, CA) |