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Table 2 Immunohistochemistry: Antibodies detecting Gd or GdA were published by Jeschke et al. 2006[26] and Jeschke et al. 2005[32], respectively

From: Immunosuppressive Glycodelin A is an independent marker for poor prognosis in endometrial cancer

Antibody Host/clonality Epitope retrieval Blocking Dilution/incubation Negative control Reaction system
Gd [32] Rabbit/polyclonal Not performed Reagent 1 (5 min) 1: 800 (diluted in Dako antibody diluent)/o.n. (4°C) Rabbit pre-immune serum (Dako) Vectastain elite (rabbit IgG) kit (Burlingame, CA)
GdA [26] Mouse/monoclonal Citrate buffer (5 min, pressure cooker) 1.5% horse serum (20 min) 1:2000 (diluted in Dako antibody diluent)/o.n. (4°C) Mouse pre-immune serum (Dako) Vectastain elite (mouse IgG) kit (Burlingame, CA)