Gd mRNA (PAEP) was detected in endometrial cancer tissue by in situ hybridization. Representative microphotographs of Glycodelin (Gd) mRNA (PAEP, progestagen-associated endometrial protein) as detected by in situ hybridization in different histological subtypes (A-C) of endometrial cancer tissue are shown. Samples were treated with an antisense riboprobe recognizing Gd mRNA (A-C) or with the complementary sense riboprobe as a negative control (insert in A), respectively. Mean optical density of Gd mRNA signal has been quantified in a semi-automated manner and Gd positive pixels were determined by KSRun software. Gd mRNA positivity in dependence of histological subtype (D), FIGO stage (E) and grading (F) is illustrated using box plot diagrams. Scale bar in C represents 100 μm and refers to A-C.