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Table 1 Oligonucleotide Sequences for (q)RT-PCR and miR-143

From: Up-regulated microRNA-143 in cancer stem cells differentiation promotes prostate cancer cells metastasis by modulating FNDC3B expression

  Name Sequence
Oct4 Sense primer 5-TCCCATGCATTCAAACTGAGGTGC-3
  Antisense primer 5-AACTTCACCTTCCCTCCAACCAGT-3
Sox2 Sense primer 5-TGGACAGTTACGCGCACAT-3
  Antisense primer 5-CGAGTAGGACATGCTGTAGGT-3
Nanog Sense primer 5-AAGGTCCCGGTCAAGAAACAG-3
  Antisense primer 5-CTTCTGCGTCACACCATTGC-3
GAPDH Sense primer 5-GCACCGTCAAGGCTGAGAAC-3
  Antisense primer 5-TGGTGAAGACGCCAGTGGA-3
miR-143 Stem-loop 5-GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGA-Ctgagcta-3
U6 Sense primer 5-ACACTCCAGCTGGGTGAGATGAAGCACTGTAG-3
  Antisense primer 5-CTCAACTGGTGTCGTGGA-3
FNDC3B Sense primer 5-GCTTCGGCAGCACATATACTAAAAT-3
  Antisense primer 5-CGCTTCACGAATTTGCGTGTCAT-3
miR-143 Sense primer 5-GGGACAGACACCCGTTTTGA-3
  Antisense primer 5-GTGTTGCCCACGGTAATGCT-3
  inhibitor 5-GAGCUACAGUGCUUCAUCUCA-3
  negative control 5-UUCUCCGAACGUGUCACGUTT-3