The down-regulation of miR-143 inhibited prostate cancer cells migration and invasion in vitro. A, The down-regulation of miR-143 in PC-3, PC-3-M and LNCaP cells by siRNA was verified by qRT-PCR. B-C, The migration of cells in which miR-143 was down-regulated was analyzed by a wound-healing assay and transwell assays. D, The invasive properties of the indicated cells were evaluated in an invasion assay using a transwell insert coated with Matrigel. Penetrated cells were counted and analyzed with a histogram (* P < 0.05, ** P < 0.01, Student’s t test).