Figure 1

MiR-143 expression was progressively increased during PC-3 spheres differentiation. A, The relative expression of Oct4, Sox2 and Nanog were analyzed in PC-3 spheres, adherent cells, and re-adherent cells on days 2, and 4 by qRT-PCR (ANOVA). B, The relative expression of miR-143 in PC-3 spheres and adherent cells was determined by qRT-PCR (Student’s t test). C, The relative expression of miRNAs were analyzed on days 0, 2, and 4 after PC-3 sphere cells were digested into single cells for re-adherent culture by qRT-PCR (ANOVA) **p < 0.01.