Methylation status of LINE-1. Methylation levels of four successive CpG sites in LINE-1 were analyzed in hepatoblastomas (T), paired adjacent normal liver tissues (A), and three normal control livers (C) by pyrosequencing. The normal controls were analyzed in triplicate and the average values were plotted with standard deviations. The average values of methylation (%) are shown for each sample groups. The asterisks indicated significant differences (p < 0.0167). Bonfferoni correction was applied for this statistical analysis.