Pharmacological genome demethylation upregulated Gls2 expression in human liver and colon cancer cells. (A) Schematic structure of the Gls2 CpG island (CGI), with the exon 1 and MSP and BGS regions indicated. Each short vertical line represents one CG site. The position of MSP and BGS regions was marked with arrows. MSP, methylation- specific PCR; BGS, bisulfite genome sequencing. Gls2 expression in human liver (B) and colon (C) cancer cells after demethylation treatment with 5 μM Aza for three days. Data was presented as mean ± SD from three independent measurements.