Eugenol inhibits tumor growth and modulates gene expression in vivo. Breast cancer xenografts were created by injecting MDA-MB-231 cells subcutaneously into nude mice. When tumors grew, eugenol was given i.p. at a dose of 100 mg/kg. Control animals were treated with DMSO. (A) Tumor growth. Data are presented as means ± S.D. (B) Following the treatments, tumors were excised and protein extracts were prepared and used for immunoblotting analysis using the indicated antibodies.