Figure 5
From: Eugenol triggers apoptosis in breast cancer cells through E2F1/survivin down-regulation
Eugenol inhibits breast cancer cell proliferation and up-regulates p21 WAF1 . (A) Sub-confluent cells (2–4.103) were either sham-treated or challenged with eugenol (2 μM) for the indicated periods of time, and cell proliferation rate was determined using the Real-Time Cell Electronic Sensing System. (B) MDA-MB-231 and MCF7 cells were treated with eugenol (2 μM) for the indicated periods of time, and then cell lysates were prepared and 50 μg of proteins were used for western blot analysis utilizing the indicated antibodies.