FACS analysis and Western blot analysis. (a) FACS analysis was performed to evaluate the effect of various treatments on iNOS expression in U251 glioma cells and 5310 glioma xenografts. (b) Western blot analysis showing the effect of various treatments on iNOS protein expression in U251 and 5310 cells. (c) Quantification of Western blot analysis results using Image J software. Columns represent mean (n = 3). Error bars represent ± SEM. *p < 0.05 vs. control.