Western blot analysis of U251 and 5310 glioma cells subjected to various treatments. (a) Western blot analysis showing the effect of transfections with scrambled vector (SV-sh), MMP-9 plasmid shRNA (M-sh), uPAR plasmid shRNA (U-sh), or MMP-9 + uPAR plasmid shRNA (MU-sh) on the expression levels of several proteins associated with α9β1-mediated cell migration in U251 and 5310 glioma cells (n = 3). (b) Quantification of Western blot analysis results using Image J software. Columns represent mean (n = 3). Error bars represent ± SEM. *p < 0.05 vs. control.