Migration potential of U251 glioma cells reduced after treatment with iNOS inhibitor. (a) U251 glioma cells were cultured in six-well plates and transfected with full-length MMP-9 (M-fl) and uPAR (U-fl) plasmids. 72 hrs after transfection, a straight scratch was made in individual wells with a 200 μL pipette tip. This point was considered to be the 0 hr, and the width of the wound was photographed under a microscope. At this point, additional wells of a six-well plate containing U251 cells from control, M-fl and U-fl treatments were subjected to treatment with L-NAME, an inhibitor of iNOS at 1 mM concentration. At the 21st hr, the cells were checked for wound healing and again photographed under a microscope. Bar graph represents the quantification of wound healing assay results. Columns represent mean (n = 3). Error bars represent ± SEM. *p < 0.05 vs. control (Ctrl). #
p < 0.05 vs. M-fl. ##
p < 0.05 vs. U-fl. (b) U251 spheroids were transfected with M-fl and U-fl plasmids. A few spheroids from each group were treated with L-NAME. Bar graph represents the quantification of cell migration from the spheroids. Columns represent mean (n = 3). Error bars represent ± SEM. *p < 0.05 vs. M-fl. #
p < 0.05 vs. U-fl.