Figure 4

LF-MF raises survival rate and inhibit tumor proliferation in vivo . (A) Statistical analysis of survival after intravenous challenge of C57BL/6 mice with B16-F10 cells, and treated with LF-MF. Mice were treated byLF-MF for 43 days, 2 h/day; (B) HE of mice lung in different groups. (C) Immunohistochemistry analyzes the expression of Ki-67 in tumors of tumor-bearing mice and in lungs of normal mice. (D) Proportion of Ki-67 positive cells in different groups. Five different microscope fields of each group were examined for Figure 4B and C, and cells in five different microscope fields of each Ki-67 staining tissue were used to calculate positive cells in Figure 4D.