Table 6 QPCR primers
Gene | Accession number | Forward primer 5′ > 3′ | Reverse primer 5′ > 3′ | Amplicon size (bp) |
|---|---|---|---|---|
Genes of interest | ||||
CCL27 | NM_006664 | GACTGTCACCTCCAGGCTTT | TCTCTTGGTGCTCAAACCAC | 100 |
K17 | NM_000422 | CTGGAGCAGGAGATTGCCAC | GGGTGGTCACCGGTTCTTTC | 88 |
MMP1 | NM_002421 | AGGTCTCTGAGGGTCAAGCA | CTGGTTGAAAAGCATGAGCA | 111 |
MMP3 | NM_002422 | TGCTTTGTCCTTTGATGCTG | GGAAGAGATGGCCAAAATGA | 135 |
MMP9 | NM_004994 | CCTGGAGACCTGAGAACCAA | ATTTCGACTCTCCACGCATC | 103 |
MMP10 | NM_002425 | GTGGAGTTCCTGACGTTGGT | TCAATGGCAGAATCAACAGC | 130 |
PI3 | NM_002638 | GACTGCCCAGGAATCAAGAA | CAGCAGGGACTTAGGACCAG | 148 |
SERPINB4 | NM_002974 | CAAAGGGCAGTGGGAGAATA | CCTCCAGCAAGGCAAAATTA | 131 |
TUBB3 | NM_006086 | CTCAGGGGCCTTTGGACATC | CCCTCCGTGTAGTGACCCTT | 96 |
Reference genes | ||||
ARPC2 | NM_005731 | TCCGGGACTACCTGCACTAC | GGTTCAGCACCTTGAGGAAG | 96 |
BAT3 | NM_004639 | AAGAGACGCAAGACGATGCAG | TGTAGCTCTCCTGAACCTCTGG | 151 |
RPS29 | NM_001032 | TATGTGCCGCCAGTGTTTCC | TGCCCCGGATAATCCTCTGA | 92 |
ZNF410 | NM_021188 | GCTGTGGTAAGCAGTTTACTACAG | CTTGGGCTTCACAAAGGAAAGG | 90 |