Skip to main content

Advertisement

Figure 2 | BMC Cancer

Figure 2

From: Phosphatidylinositol 3-Kinase dependent upregulation of the epidermal growth factor receptor upon Flotillin-1 depletion in breast cancer cells

Figure 2

Expression of the epidermal growth factor receptor family members in MCF7 cells depleted of flotillin-1 or flotillin-2. Expression of the epidermal growth factor receptor family members EGFR, ErbB2 and ErbB3 in MCF7 cells depleted of flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B). (A) Western blot using specific antibodies. GAPDH was detected to show equal loading. (B-D) Densitometric quantification. The signals were normalized to GAPDH. Bars represent the mean ± SD of three independent experiments. Statistical analysis using one-way ANOVA. *, p < 0.05; **, p < 0.01. (E) Staining of endogenous EGFR in MCF7 cells depleted of flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B). Scale bar: 20 μm. (F) Quantitative real-time PCR analysis showing the relative mRNA level of EGFR in MCF7 control and flotillin-1 (sh-F1-A/B) or flotillin-2 (sh-F2-A/B) knockdown cells using two different primer pairs. The expression was normalized to GAPDH and Rpl13a. Bars represent the mean ± SD of three independent experiments. Statistical analysis using one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Back to article page