Immunohistochemical analysis of ZEB-1 and E-cadherin expression. ZEB-1 antibody was purchased from SANTA CRUZ BIOTECHNOLOGY, Inc. and E-cadherin antibody was purchased from DAKO JAPAN. Images from representative cases are shown: case 1 (a - d) and case 2 (e. f). Case 1 was classified as >10% ZEB-1 positive. a. Positive expression of ZEB-1 in cellular nuclei in HCC. b. Reduced expression of E-cadherin in HCC cells. c. ZEB-1 expression is undetectable in noncancerous liver cells. d. E-cadherin expression was observed in the cell membrane in noncancerous liver cells. Case 2 was classified as ZEB-1 negative. e. ZEB-1 was not detected in the cell nuclei in HCC cells. f. E-cadherin expression was preserved in the cell membrane in HCC cells.