Effects of Cs, NAC and TEMPOL on PEITC-induced cell death. Cells were treated with 3 or 10 μM of PEITC (P3 or P10) in combination with Cs (cyclosporine) (50 nM) for 24 h (A and B). Another experiments, cells were treated with NAC (2 mM) or TEMPOL (0.5 mM, TEM) for 3, 24 and 48 h (C and D). Cell viability was assayed. Each bar or each value represents the mean ± SEM of three experiments. Significantly different from control group, *p < 0.05. and significantly different from PEITC treated group, #p <0.05.