Figure 6

PEITC induced the mobilization of intracellular Ca ²⁺ . Cultured cells were treated with 3 or 10 μM of PEITC (P3 or P10) with NAC (2 mM) for 1 h. The mobilization of Ca²⁺ into cytosol was analyzed by fluorescence staining using Fluoforte dye with excitation and emission wavelength of 485 and 535 nm, respectively. Representatives of images in each condition are shown (A & B). The integrated optical density (IOD) of fluorescence in each condition was analyzed (C & D) from 10 images of two experiments. Each bar represents the mean ± SEM. Significantly different from control, *p < 0.05 and significantly different from PEITC treated group, #p <0.05.