Figure 5

Effect of PEITC on ROS production and GSH depletion. KKU-M214 (A & C) and Chang cells (B & D) were incubated with PEITC at indicated concentrations 3 and 10 μM (P3 & P10) with or without TEMPOL (0.5 mM TEM) or NAC (2 mM). ROS formation was quantified by dihydroethidium method after incubation for 90 min (A & B) and cellular GSH (C & D) was assayed at 3 h after incubation. Each bar represents the mean ± SEM of three experiments. Significantly different from control, *p < 0.05, and significantly different from PEITC treated group, #p < 0.05.