Figure 2

PEITC-induced apoptosis and release of proapoptogenic proteins. KKU-M214 and Chang cells were treated with PEITC 1, 3 or 10 μM (P1, P3 or P10, respectively) for 3 or 6 h. The number of apoptotic cells was assessed by AO/EB method at 3 h of incubation (A). Each bar represents the mean ± SEM of three experiments. Significantly different from control, *p < 0.05. Immunoblot analysis was performed using 30 μg of protein and cytochrome c, Bax, Bcl-xl, AIF and p53 were detected by specific antibodies. β-actin was used as loading control (B & C). Value indicates the relative protein intensity as a ratio to the corresponding vehicle-treated control. Each experiment was done at least twice with similar results, and the representative bands from one experiment are shown.