Receiver Operating Characteristics (ROC) analysis of EDIM-Apo10 and EDIM-TKTL1 score in OSCC (n = 50) compared with healthy individuals (n = 74), and interactive dot diagrams. The true positive rates (sensitivity) are plotted in function of the false positive rate (100-specificity) for measurement of the cut-off point: ROC analysis for the diagnosis of primary or recurrent OSCC shows calculated cut-off value with highest diagnostic accuracy (arrows) of EDIM-Apo10 (a) and EDIM-TKTL1 (b) score (a, EDIM-Apo10 score >109: sensitivity 90.0%, 95% CI 78.2–96.7%, specificity 94.6%, 95% CI 86.7–98.5%; b, EDIM-TKTL1 score >117: sensitivity 92.0%, 95% CI 80.8–97.8%, specificity 95.9%, 95% CI 88.6–99.2%). Dotted lines show 95% CI. OSCC, oral squamous cell carcinoma. In the interactive dot diagrams (part of ROC curve analysis, c, d) the data of healthy controls and OSCC group are displayed as dots on two vertical axes. The horizontal line indicates the cut-off points with the best separation/highest accuracy (minimal false negative and false positive results) between healthy controls and OSCC group. The corresponding test characteristics sensitivity and specificity are shown above.