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Figure 3 | BMC Cancer

Figure 3

From: Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival

Figure 3

Effect of DSS1 over-expression on sensitivity to DNA damage. Effect of DSS1 over-expression on drug-induced DNA injury was measured using the alkaline comet assay after treatment of cells with CPT (50 μM) for 3 hr. DMSO was used as a solvent control. The DNA content pattern observed under fluorescent microscopy is shown (left), and the DNA damage rate was measured as the tail moment in the fluorescence image using CometScore software. DSS-over-expressing MCF7 (A) or MDA-MB-231 (B) cells were compared with GFP-expressing controls. Data obtained by counting more than 100 cells are shown as a graph, which represents more than three independent experiments (right). Statistical significance was calculated using Student’s t-test, with **P < 0.01 as significant. Scale bar indicates 100 μm.

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