Table 1 Oligonucleotides used for the qRT-PCR analysis
From: Restoration of WNT4 inhibits cell growth in leukemia-derived cell lines
Gene name | Sequence accession number | Primer sequence | Primer location (Exon) | Prod. length | T°a | |
|---|---|---|---|---|---|---|
WNT4 ORF | Wingless-type MMTV integration site family, member 4 | NM_030761 | F GGCACCATGAGTCCCCGCTCG | 99–119 (1) | 1055 | 60 |
R GCAGGGCTAGGCAGGCGGTCA | 1158–1178 (5) | |||||
WNT4 | Wingless-type MMTV integration site family, member 4 | NM_030761 | F GGAACTGCTCCACACTCGACTC | 364–385 (3) | 259 | 60 |
R CGCACATCCACAAACGACTGT | 602–622 (4) | |||||
MYC | V-myc myelocytomatosis viral oncogene homolog (avian) | NM_002467.4 | F CCAGCGCCTTCTCTCCGTC | 1208–1226 (2) | 302 | 60 |
R GGGAGGCGCTGCGTAGTTGT | 1490–1509 (3) | |||||
JUN | Jun proto-oncogene | NM_002228.3 | F TGGAAAGTACTCCCCTAACCT | 2786–2806 (1) | 250 | 60 |
R CTGAAACATCGCACTATCCTT | 3015–3035 (1) | |||||
FOSL1/FRA1 | FOS-like antigen 1 | NM_005438.3 | F AGGAACCGGAGGAAGGAACTG | 554–574 (3) | 199 | 60 |
R TGCCACTGGTACTGCCTGTGT | 732–752 (4) | |||||
AXIN2 | Axin 2 | NM_004655.3 | F AAAAAGGGAAATTATAGGTATTAC | 2678–2701 (10/11) | 277 | 54 |
R CGATTCTTCCTTAGACTTTG | 2935–2954 (11) | |||||
CCND1 | cyclin D1 | NM_053056.2 | F CCCCAACAACTTCCTGTCCTAC | 866–887 (4) | 236 | 60 |
R GCCCTCAGATGTCCACGTC | 1083–1101 (5) | |||||
SURVIVIN/BIRC5 | Homo sapiens baculoviral IAP repeat containing 5 | NM_001012271.1 | F TGAGCTGCAGGTTCCTTATCTG | 1057–1078 (5) | 234 | 60 |
R GAATGGCTTTGTGCTTAGTTTT | 1269–1290 (5) | |||||
RPL32 | Ribosomal protein L32 | NM_000994.3 | F GACTTGACAACAGGGTTCGTAG | 213–234 (3) | 320 | 60 |
R ATTTAAACAGAAAACGTGCACA | 511–532 (4) | |||||
RPS18 | Ribosomal protein S18 | NM_022551.2 | F CGATGGGCGGCGGAAAA | 105–121 (2) | 283 | 58 |
R CAGTCGCTCCAGGTCTTCACGG | 366–387 (5) | |||||
ACTB | Beta Actin | NM_001101.3 | F TCCGCAAAGACCTGTACG | 950–967 (5) | 298 | 60 |
R AAGAAAGGGTGTAACGCAACTA | 1226–1247 (6) |