Restoration of WNT4 in BJAB cells does not promote apoptosis, but modulates the cell cycle. A) Percentage of apoptosis was determined by using Annexin-V-Fluos/Propidium Iodide in BJAB-Tet-WNT4 cells after 48-h incubation in the presence or absence of Doxycycline (Doxy). We also included parental BJAB and BJAB-Tet cells as control. A total of 20,000 events were counted. B) Parental BJAB, BJAB-Tet, and BJAB-Tet-WNT4 cells were cultured in presence of Doxy for 48 h. Afterward, flow cytometric analysis of the DNA content was conducted to determine cell populations in the different cell-cycle phases. Dotplots and histograms were obtained with Attune Cytometric Software ver 2.1.0, G1 and G2 populations are shown. C) Histograms obtained by analyzing cell cycle with FlowJo software. A total of 20,000 events in gated singlets were counted.