Figure 3
GAD1 knockdown cells suppress translocation of β-catenin to the nucleus and MMP7 activation. a Immunoblotting analysis of β-catenin in the nucleus of shGAD1 cells. The expression of β-catenin in the nucleus of shGAD1 cells has decreased markedly compared with that in mock cells. β-catenin expression in the cytoplasm does not differ significantly between shGAD1 cells and mock cells. b Quantification of MMP7 mRNA levels in shGAD1 cells by qRT-PCR analysis. MMP7 mRNA is significantly down-regulated in shGAD1 cells compared with mock cells. c Casein zymography analysis of MMP7 activity in shGAD1. Cell culture media are collected and concentrated, MMP7 activity is analyzed by casein zymography. MMP7 secretion is decreased significantly in shGAD1 cells compared with mock cells.