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Table 1 RKIP-related proteins identified with ESI-Q-TOF MS/MS

From: Mapping the interactome of overexpressed RAF kinase inhibitor protein in a gastric cancer cell line

UniProt ID Protein name Protein mass (Da) Score Matching peptides Protein function
I. Metabolic enzymes
P53618 Coatomer subunit beta 108,241 89 3 Mediate biosynthetic protein transport
P14618 Pyruvate kinase isozymes M1/M2, isoform M2 58,470 604 40 Glycolytic enzyme
P00966 Argininosuccinate synthase 46,791 142 6 ATP binding
P00558 Phosphoglycerate kinase 1 44,992 102 3 Glycolytic enzyme
P08195 4F2 cell-surface antigen heavy chain, isoform 2 68,180 56 2 Function of light chain amino-acid transporters
P06733 Alpha-enolase 47,481 517 25 Multifunctional enzyme
P04406 Glyceraldehyde-3-phosphate dehydrogenase 36,201 769 80 Glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities
P07195 L-lactate dehydrogenase B chain 36,900 74 5 L-lactate dehydrogenase activity
P00338 L-lactate dehydrogenase A chain, isoform 1 36,950 43 4 L-lactate dehydrogenase activity
P40926 Malate dehydrogenase, mitochondrial 35,937 49 3 L-malate dehydrogenase activity
P46940 Ras GTPase-activating-like protein IQGAP1 189,761 55 3 Promote neurite outgrowth
P68104 Elongation factor 1-alpha 1 50,451 190 11 Protein biosynthesis
P02786 Transferrin receptor protein 1 85,274 74 3 Molecular transducer activity
II. Molecular chaperone
P14625 Endoplasmin 92,696 192 18 Molecular chaperone ATPase activity
P07900 Heat shock protein HSP 90-alpha, isoform 2 85,006 280 24 Stress related, chaperone
P08238 Heat shock protein HSP 90-beta 83,554 276 25 Stress related, chaperone
P11142 Heat shock cognate 71 kDa protein, isoform 1 71,082 398 16 Stress related, chaperone
P38646 Stress-70 protein, mitochondrial 73,920 253 7 Related with cellular proliferation and aging
P10809 60 kDa heat shock protein, mitochondrial 61,187 723 22 Stress related, chaperone
P53992 Protein transport protein Sec24C 119,789 61 5 Component of the COPII coat
P50454 Serpin H1 46,525 133 3 Chaperone in the biosynthetic pathway of collagen
III. Biological oxidation-related proteins
O00299 Chloride intracellular channel protein 1 27,248 78 3 Voltage-gated chloride channel activity, redox-regulated
Q06830 Peroxiredoxin-1 22,324 51 7 Redox regulation
IV. Signal transduction
O43390 Heterogeneous nuclear ribonucleoprotein R, isoform 1 71,184 63 2 mRNA processing, ribonucleoprotein
P52597 Heterogeneous nuclear ribonucleoprotein F 45,985 110 2 mRNA processing, ribonucleoprotein
P04083 Annexin A1 38,918 454 24 Signal transduction, calcium ion binding
P12429 Annexin A3 36,524 53 4 Signal transduction, calcium ion binding
P08758 Annexin A5 35,971 50 10 Signal transduction, calcium ion binding
P62258 14-3-3 protein epsilon 29,326 136 6 Anti-apoptosis, signal transduction, transcription factor binding
P23396 40S ribosomal protein S3 26,842 122 9 NF-KappaB binding
Q99623 Prohibitin-2 33,276 38 5 Mediate transcriptional repression
V. Cytoskeleton-related proteins
O75369 Filamin-B, isoform 1 280,157 91 1 Connects cell membrane constituents to the actin cytoskeleton
P21333 Filamin-A, isoform 2 283,301 91 3 Actin binding
P18206 Vinculin, isoform 1 124,292 668 30 Actin filament-binding protein
P04264 Keratin, type II cytoskeletal 1 66,170 89 4 Cytoskeleton of Intermediate filaments
P05787 Keratin, type II cytoskeletal 8 53,671 156 11 Cytoskeleton of Intermediate filaments
Q04695 Keratin, type I cytoskeletal 17 48,361 221 10 Cytoskeleton of Intermediate filaments
P05783 Keratin, type I cytoskeletal 18 48,029 547 35 Cytoskeleton of Intermediate filaments
Q15149 Plectin-1, isoform 1 533,462 73 11 Actin binding
P35579 Myosin-9, isoform 1 227,646 62 10 Play a role in cytokinesis, secretion
P68371 Tubulin beta-2C chain 50,255 245 19 The major constituent of microtubules
P07437 Tubulin beta chain 50,095 309 25 The major constituent of microtubules
P60709 Actin, cytoplasmic 1 42,058 617 36 Involved in various types of cell motility
P52907 F-actin-capping protein subunit alpha-1 33,073 61 3 Actin binding
P04075 Fructose-bisphosphate aldolase A 39,851 141 7 Actin binding
P08670 Vimentin 53,619 574 17 Cell motility, structural constituent of cytoskeleton
Q9NYL9 Tropomodulin-3 39,741 85 9 Actin binding
VI. Protease-related proteins
P53396 ATP-citrate synthase 121,674 225 7 Enzyme regulator activity
P55072 Transitional endoplasmic reticulum ATPase 89,950 42 3 Necessary for the formation of transitional endoplasmic reticulum
Q08J23 tRNA (cytosine-5-)-methyl transferase NSUN2 87,214 78 2 RNA methyltransferase
P06576 ATP synthase subunit alpha, mitochondrial 59,828 158 9 Mitochondrial membrane ATP synthase
P60842 Eukaryotic initiation factor 4A-I 46,357 169 7 ATP-dependent RNA helicase
P55786 Puromycin-sensitive aminopeptidase 103,895 117 3 Involved in proteolytic events essential for cell growth and viability
P50395 Rab GDP dissociation inhibitor beta 51,095 78 3 Rad GDP-dissociation inhibitor activity
P42330 Aldo-keto reductase family 1 member C3 37,229 35 2 Aldo-keto reductase activity
P63244 Guanine nucleotide-binding protein subunit beta-2-like 1 35,511 112 3 Up-regulation of the activity of kinases
P61289 Proteasome activator complex subunit 3, isoform 1 29,602 77 2 Endopeptidase activator activity
P09211 Glutathione S-transferase P 23,569 56 30 Glutathione transferase activity
P13639 Elongation factor 2 96,246 240 9 Catalyzes the GTP-dependent ribosomal translocation
Q15029 116 kDa U5 small nuclear ribonucleoprotein component 110,336 130 6 Component of the U5 snRNP complex
P10696 Alkaline phosphatase, placental-like 57,626 140 8 Alkaline phosphatase activity
Q99714 3-hydroxyacyl-CoA dehydrogenase type-2, isoform 1 27,134 108 3 Mitochondrial tRNA maturation
Q08211 ATP-dependent RNA helicase A 142,181 264 12 Transcriptional activator
P41252 Isoleucyl-tRNA synthetase, cytoplasmic 145,718 94 9 Isoleucine-tRNA ligase activity
P26640 Valyl-tRNA synthetase 141,642 78 4 ATP binding
VII. Others
Q13263 Transcription intermediary factor 1-beta, isoform 1 90,261 59 3 Nuclear corepressor for KRAB domain-containing zinc finger proteins
Q14974 Importin subunit beta-1 98,420 84 2 Protein domain specific binding
P26641 Elongation factor 1-gamma 50,429 65 2 Unknown
P21796 Voltage-dependent anion-selective channel protein 1 30,868 78 3 Voltage-gated anion channel activity
P61026 Ras-related protein Rab-1A, isoform 1 22,755 45 1 Involved in vesicular trafficking and neurotransmitter release.
Q00610 Clathrin heavy chain 1, isoform 1 193,620 36 3 The polyhedral coat of coated pits and vesicles
Q14683 Structural maintenance of chromosomes protein 1A 143,771 221 6 Involved in chromosome cohesion