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Table 1 RKIP-related proteins identified with ESI-Q-TOF MS/MS

From: Mapping the interactome of overexpressed RAF kinase inhibitor protein in a gastric cancer cell line

UniProt ID

Protein name

Protein mass (Da)

Score

Matching peptides

Protein function

I. Metabolic enzymes

P53618

Coatomer subunit beta

108,241

89

3

Mediate biosynthetic protein transport

P14618

Pyruvate kinase isozymes M1/M2, isoform M2

58,470

604

40

Glycolytic enzyme

P00966

Argininosuccinate synthase

46,791

142

6

ATP binding

P00558

Phosphoglycerate kinase 1

44,992

102

3

Glycolytic enzyme

P08195

4F2 cell-surface antigen heavy chain, isoform 2

68,180

56

2

Function of light chain amino-acid transporters

P06733

Alpha-enolase

47,481

517

25

Multifunctional enzyme

P04406

Glyceraldehyde-3-phosphate dehydrogenase

36,201

769

80

Glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities

P07195

L-lactate dehydrogenase B chain

36,900

74

5

L-lactate dehydrogenase activity

P00338

L-lactate dehydrogenase A chain, isoform 1

36,950

43

4

L-lactate dehydrogenase activity

P40926

Malate dehydrogenase, mitochondrial

35,937

49

3

L-malate dehydrogenase activity

P46940

Ras GTPase-activating-like protein IQGAP1

189,761

55

3

Promote neurite outgrowth

P68104

Elongation factor 1-alpha 1

50,451

190

11

Protein biosynthesis

P02786

Transferrin receptor protein 1

85,274

74

3

Molecular transducer activity

II. Molecular chaperone

P14625

Endoplasmin

92,696

192

18

Molecular chaperone ATPase activity

P07900

Heat shock protein HSP 90-alpha, isoform 2

85,006

280

24

Stress related, chaperone

P08238

Heat shock protein HSP 90-beta

83,554

276

25

Stress related, chaperone

P11142

Heat shock cognate 71 kDa protein, isoform 1

71,082

398

16

Stress related, chaperone

P38646

Stress-70 protein, mitochondrial

73,920

253

7

Related with cellular proliferation and aging

P10809

60 kDa heat shock protein, mitochondrial

61,187

723

22

Stress related, chaperone

P53992

Protein transport protein Sec24C

119,789

61

5

Component of the COPII coat

P50454

Serpin H1

46,525

133

3

Chaperone in the biosynthetic pathway of collagen

III. Biological oxidation-related proteins

O00299

Chloride intracellular channel protein 1

27,248

78

3

Voltage-gated chloride channel activity, redox-regulated

Q06830

Peroxiredoxin-1

22,324

51

7

Redox regulation

IV. Signal transduction

O43390

Heterogeneous nuclear ribonucleoprotein R, isoform 1

71,184

63

2

mRNA processing, ribonucleoprotein

P52597

Heterogeneous nuclear ribonucleoprotein F

45,985

110

2

mRNA processing, ribonucleoprotein

P04083

Annexin A1

38,918

454

24

Signal transduction, calcium ion binding

P12429

Annexin A3

36,524

53

4

Signal transduction, calcium ion binding

P08758

Annexin A5

35,971

50

10

Signal transduction, calcium ion binding

P62258

14-3-3 protein epsilon

29,326

136

6

Anti-apoptosis, signal transduction, transcription factor binding

P23396

40S ribosomal protein S3

26,842

122

9

NF-KappaB binding

Q99623

Prohibitin-2

33,276

38

5

Mediate transcriptional repression

V. Cytoskeleton-related proteins

O75369

Filamin-B, isoform 1

280,157

91

1

Connects cell membrane constituents to the actin cytoskeleton

P21333

Filamin-A, isoform 2

283,301

91

3

Actin binding

P18206

Vinculin, isoform 1

124,292

668

30

Actin filament-binding protein

P04264

Keratin, type II cytoskeletal 1

66,170

89

4

Cytoskeleton of Intermediate filaments

P05787

Keratin, type II cytoskeletal 8

53,671

156

11

Cytoskeleton of Intermediate filaments

Q04695

Keratin, type I cytoskeletal 17

48,361

221

10

Cytoskeleton of Intermediate filaments

P05783

Keratin, type I cytoskeletal 18

48,029

547

35

Cytoskeleton of Intermediate filaments

Q15149

Plectin-1, isoform 1

533,462

73

11

Actin binding

P35579

Myosin-9, isoform 1

227,646

62

10

Play a role in cytokinesis, secretion

P68371

Tubulin beta-2C chain

50,255

245

19

The major constituent of microtubules

P07437

Tubulin beta chain

50,095

309

25

The major constituent of microtubules

P60709

Actin, cytoplasmic 1

42,058

617

36

Involved in various types of cell motility

P52907

F-actin-capping protein subunit alpha-1

33,073

61

3

Actin binding

P04075

Fructose-bisphosphate aldolase A

39,851

141

7

Actin binding

P08670

Vimentin

53,619

574

17

Cell motility, structural constituent of cytoskeleton

Q9NYL9

Tropomodulin-3

39,741

85

9

Actin binding

VI. Protease-related proteins

P53396

ATP-citrate synthase

121,674

225

7

Enzyme regulator activity

P55072

Transitional endoplasmic reticulum ATPase

89,950

42

3

Necessary for the formation of transitional endoplasmic reticulum

Q08J23

tRNA (cytosine-5-)-methyl transferase NSUN2

87,214

78

2

RNA methyltransferase

P06576

ATP synthase subunit alpha, mitochondrial

59,828

158

9

Mitochondrial membrane ATP synthase

P60842

Eukaryotic initiation factor 4A-I

46,357

169

7

ATP-dependent RNA helicase

P55786

Puromycin-sensitive aminopeptidase

103,895

117

3

Involved in proteolytic events essential for cell growth and viability

P50395

Rab GDP dissociation inhibitor beta

51,095

78

3

Rad GDP-dissociation inhibitor activity

P42330

Aldo-keto reductase family 1 member C3

37,229

35

2

Aldo-keto reductase activity

P63244

Guanine nucleotide-binding protein subunit beta-2-like 1

35,511

112

3

Up-regulation of the activity of kinases

P61289

Proteasome activator complex subunit 3, isoform 1

29,602

77

2

Endopeptidase activator activity

P09211

Glutathione S-transferase P

23,569

56

30

Glutathione transferase activity

P13639

Elongation factor 2

96,246

240

9

Catalyzes the GTP-dependent ribosomal translocation

Q15029

116 kDa U5 small nuclear ribonucleoprotein component

110,336

130

6

Component of the U5 snRNP complex

P10696

Alkaline phosphatase, placental-like

57,626

140

8

Alkaline phosphatase activity

Q99714

3-hydroxyacyl-CoA dehydrogenase type-2, isoform 1

27,134

108

3

Mitochondrial tRNA maturation

Q08211

ATP-dependent RNA helicase A

142,181

264

12

Transcriptional activator

P41252

Isoleucyl-tRNA synthetase, cytoplasmic

145,718

94

9

Isoleucine-tRNA ligase activity

P26640

Valyl-tRNA synthetase

141,642

78

4

ATP binding

VII. Others

Q13263

Transcription intermediary factor 1-beta, isoform 1

90,261

59

3

Nuclear corepressor for KRAB domain-containing zinc finger proteins

Q14974

Importin subunit beta-1

98,420

84

2

Protein domain specific binding

P26641

Elongation factor 1-gamma

50,429

65

2

Unknown

P21796

Voltage-dependent anion-selective channel protein 1

30,868

78

3

Voltage-gated anion channel activity

P61026

Ras-related protein Rab-1A, isoform 1

22,755

45

1

Involved in vesicular trafficking and neurotransmitter release.

Q00610

Clathrin heavy chain 1, isoform 1

193,620

36

3

The polyhedral coat of coated pits and vesicles

Q14683

Structural maintenance of chromosomes protein 1A

143,771

221

6

Involved in chromosome cohesion