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Table 2 The antibodies used in immunohistochemical staining and Western blot

From: A synthetic dsRNA, as a TLR3 pathwaysynergist, combined with sorafenib suppresses HCC in vitro and in vivo

Antibody Producer Applications Working dilutions Positive localization
TLR3 Abcam Western blot 1:1000 cytoplasmic
1:100
NF-κB p65 Cell Signaling Technology Western blot Immunofluorescence 1:1000 cytoplasmic
1:100 nucleus
Lamin B Santa Cruz Biotechnology, Western blot 1:100 nucleus
α-tubulin Santa Cruz Biotechnology, Western blot   cytoplasmic
Survivin Santa Cruz Biotechnology Western blot 1:1000 cytoplasmic
PCNA Santa Cruz Biotechnology Western blot 1:1000 nucleus
BCL-2 Santa Cruz Biotechnology Western blot 1:1000 cytoplasmic
β-actin Santa Cruz Biotechnology Western blot 1:2500  
Goat anti-rabbit IgG Cell Signaling Technology Western blot Immunohistochemical 1:1000  
1:100
Goat anti-rabbit Multiscience Immunofluorescence 1:100  
IgG(H + L),FITC