Table 2 The antibodies used in immunohistochemical staining and Western blot
Antibody | Producer | Applications | Working dilutions | Positive localization |
|---|---|---|---|---|
TLR3 | Abcam | Western blot | 1:1000 | cytoplasmic |
1:100 | ||||
NF-κB p65 | Cell Signaling Technology | Western blot Immunofluorescence | 1:1000 | cytoplasmic |
1:100 | nucleus | |||
Lamin B | Santa Cruz Biotechnology, | Western blot | 1:100 | nucleus |
α-tubulin | Santa Cruz Biotechnology, | Western blot | cytoplasmic | |
Survivin | Santa Cruz Biotechnology | Western blot | 1:1000 | cytoplasmic |
PCNA | Santa Cruz Biotechnology | Western blot | 1:1000 | nucleus |
BCL-2 | Santa Cruz Biotechnology | Western blot | 1:1000 | cytoplasmic |
β-actin | Santa Cruz Biotechnology | Western blot | 1:2500 | |
Goat anti-rabbit IgG | Cell Signaling Technology | Western blot Immunohistochemical | 1:1000 | |
1:100 | ||||
Goat anti-rabbit | Multiscience | Immunofluorescence | 1:100 | |
IgG(H + L),FITC |