Figure 5

PPP treatment inhibits the growth of TP53 wild type carcinoma xenografts. (A). HCT-8 cells were injected subcutaneously in athymic mice for xenograft formation. Once the xenografts were formed, the mice were treated either with PPP (50 mg/kg) in the treatment group or saline in the control group through oral gavage, twice per week. The tumor volumes from the same group of mice were grouped and presented as mean ± SD. **, p < 0.01. (B). The representative mice bearing xenografts were shown at necropsy with saline-treated mouse on the left and PPP-treated mouse on the right. (C). The preventative xenograft tumors (T) from saline treated (−) and PPP treated mice (+) were subjected to western blotting for the presence of the phosphorylated and unphosphorylated IGF-1R, AKT and ERK.