Figure 7
From: Identification of the angiogenic gene signature induced by EGF and hypoxia in colorectal cancer
Scatter plot PCR array analysis of Caco-2 cells exposed to EGF alone or in combination with DMOG. Caco-2 cells were stimulated with 20 ng/mL EGF for 24 hours alone (a) or in combination with 1mM DMOG (b). Scatter plot graphs are 2-ΔCt values for genes expressed by Caco-2 and normalised against HKG ActB (β-actin), 18S rRNA, HPRT1 (hypoxanthine phosphoribosyltransferase 1) and RPL13A (60S ribosomal protein L13a). Solid lines show no change, dashed lines show ≥2-fold increase and decrease versus untreated. Genes whose expression in both treated and untreated samples was below detection limits of the array are not included. Only genes whose expression changed ≥2-fold are annotated, with annotated open circles representing the 9 genes also changed in response to hypoxia and DMOG, and annotated closed circles representing the 11 additional genes uniquely changed only in response to DMOG plus EGF. Data are from a representative array performed in duplicate using cDNA pooled from 3 different replicate experiments.