HIF-α in Caco-2 cells exposed to EGF and/or DMOG. Caco-2 cells were stimulated with 20ng/mL EGF and/or 1mM DMOG for the time periods indicated. Fold change in (a) HIF-1α and (b) HIF-2α mRNA levels were determined by Q-PCR using the 2-ΔΔCt method and are expressed relative to HKG 18S. Data are mean ± SEM from 2 representative experiments. (c) HIF-1α and HIF-2α protein was measured by Western blotting in Caco-2 cells stimulated with EGF and/or DMOG for 24 hours. α-tubulin is shown as a loading control. (d) HIF-1α protein was measured by ELISA. Data are mean ± SEM from 3 representative experiments, and were analyzed by 2-way ANOVA versus unstimulated cells: *** p < 0.001.